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2010, 97. 207-215. Anti-Ku70 / 80 Antibody (A34877) Rabbit polyclonal antibody to Ku70 / 80 Validated Applications: WB, IHC, IF. Tested Reactivity: Human, Mouse, Rat. View Datasheet The Ku70/80 heterodimer binds to DNA ends and attracts other proteins involved in the non-homologous end-joining (NHEJ) pathway of DNA double-strand break repair. We developed a novel assay to measure DNA binding and release kinetics using differences in Förster resonance energy transfer (FRET) of the ECFP-Ku70/EYFP-Ku80 heterodimer in soluble and DNA end bound states.

Ku70 80

The Ku70/80 heterodimer is also required for osteocalcin gene expression.Hundratusentals antikroppar är tillgängliga hos VWR. Hitta din antikropp genom att repair in mammalian cells. DNA PK consists of a heterodimeric DNA-binding subunit (Ku70/80) and an approximately 465 kDa catalytic subunit (DNA PKcs). Den Ku70/Ku80 heterodimer binder till DSB slutar, rekrytera DNA-PKcs och p53 Bindande Odla celler till 80% konfluency (cirka 24 timmar). Ku70 visas i lila, Ku80 i blått och DNA-strängen i grönt. Identifierare Ku70 / Ku80 N-terminal alfa / beta-domän.

Persson, Oscar et al. "Distribution, cellular localization, and therapeutic potential of the tumor-associated antigen Ku70/80 in glioblastoma multiforme.". Journal of Neuro-Oncology.

ENSP00000317668 ENSG00000146556 ensHS ens Ensembl

Besides sensing, Ku70/80 complex possibly tethers broken DNA ends keeping them in close vicinity for end-processing and subsequent ligation. Concomitantly, DNA-bound Ku70/80 proteins serve as a docking platform for sequential binding of C-NHEJ components and for an array of proteins mediating the DNA-damage response. The Ku70/80 heterodimer is the regulatory subunit of the DNA-dependent protein kinase (DNA-PK) and its DNA-binding activity mediates DNA double-strand breaks repair.

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Tel: 070-538 80 97 karin.stenzelius@skane.se behandling, men däremot en ökning av nukleärt Ku70-protein.

Ku70 or Ku80 overexpression Ku70 — белок, в человеческом организме кодируемый геном XRCC6. Содержание.
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Since this central position, the Ku70/80 dimer is a logical target for the disruption of the entire NHEJ pathway. Surprisingly, specific inhibitors of the Ku70/80 heterodimer are currently not available. The Ku70/80 heterodimer is the regulatory subunit of the DNA-dependent protein kinase (DNA-PK) and its DNA-binding activity mediates DNA double-strand breaks repair. Although Ku80 was recently The Ku70/80 heterodimer (Ku), the catalytic subunit of the DNA-dependent protein kinase (DNA-PKcs), DNA ligase IV (LigIV), XRCC4 and XLF form a long-range synaptic complex, in which the DNA ends The Ku70/80 crystal structure (Fig. 1B) shows that the two subunits dimerize through the central domain to form a ring capable of accommodating two turns of double-stranded DNA (approximately 14 base pairs) .

KU70.
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Additional slow migrating bands were observed in Ku70/80 (25, 50 and 100 nM) in the presence of RECQ1 (12.5 nM) (lanes 4–6 and 7–9). Fig. 5 Effect of Ku70/80 and DNA-PKcs on Trx-RAG-mediated cleavage of linear DNA. A, linear DNA substrates were incubated with Trx-RAGs, in the absence or presence of HMGB1 (50 (top gel) or 500 ng (bottom gel)), Ku70/80, and DNA-PKcs. Bottom, percentage of SE product is shown. Substrate and cleavage products are represented to the left. The Ku70/80 heterodimer protein possesses a ring-shaped structure with high affinity for DSBs. Since this central position, the Ku70/80 dimer is a logical target for the disruption of the entire NHEJ pathway.

Företagsdatabasen FDB, 2017 - SCB

This ring, consisting of intertwined strands of both Ku70 and Ku80, is lined with positively charged residues positioned to interact with Heterodimers of the 70 and 80 kDa Ku autoantigens (Ku70 and Ku80) activate the DNA‐dependent protein kinase (DNA‐PK). Mutations in any of the three subunits of this protein kinase (Ku70, Ku80 and DNA‐PKcs) lead to sensitivity to ionizing radiation (IR) and to DNA double‐strand breaks, and V(D)J recombination product formation defects.

3A, lane 3), indicating that Ku70/80 and RECQ1 can together bind the fork DNA structure.